Unit 1 Animal Cell Culture

8 lecture hours

• History of Animal Cell Culture
• Characteristics of animal cell
• regulation and nutritional requirements
• Culture Media and Growth Conditions
• Development of Primary Culture and Cell Lines
• Suspension Culture
• Characterization and maintenance of cell lines
• Common Cell Culture Contaminants
• Marker Gene Characterization
• Transfection and Transformation of Cells

Unit 2 Growth and Scale Up

5 lecture hours

• Need for scaling-up of cells for vaccine or antigen or Subject Code: 3170414 w.e.f
• AY 2018-19 pharmaceutical protein production
• Hybridoma Technology
• Cell culture reactors
• Scale-Up in suspension and monolayer cultures
• Factors affecting cell growth
• Growth Monitoring
• Mass Transfer

Unit 3 Animal Biotechnology

8 lecture hours

• Concept of transgenic animals
• Methods of transgene delivery
• Microinjection of recombinant DNA into fertilized eggs/stem cells
• Animal Pharming
• Organ Culture
• Regenerative Medicine
• Human Embryonic Stem Cell research

Unit 4 Crop Improvement

4 lecture hours

• The need of crop improvement
• Conventional methods of crop improvement
• polyploidy and clonal selection
• Green revolution in India
• Introduction to marker assisted breeding and selection

Unit 5 Plant tissue culture

8 lecture hours

• History of plant tissue culture
• plasticity and totipotency
• Laboratory setup for a typical plant tissue culture facility
• Sterilization methods used in plant tissue culture
• Types of nutrient media and plant growth regulators in plant regeneration
• Pathways for in vitro regeneration: organogenesis
• somatic and gametic 93 embryogenesis
• protoplast isolation
• culture and regeneration
• culture of other explants
• somatic hybridization
• Haploid and triploid production and their applications
• Genetic fidelity of plants raised through tissue culture
• Applications of micro-propagation
• meristem culture
• embryo rescue
• somaclonal and androclonal variations
• Application of tissue culture for crop improvement
• Methods for Plant Conservation
• synseed production
• Production of bio active secondary metabolites by plant tissue culture

Unit 6 Principles and methods of genetic transformation

8 lecture hours

• Introduction to Agrobacterium biology and biotechnology
• Mechanism of T-DNA transfer to plants and Agro infection
• A. rhizogenes and its application
• Transplastomics and its utility
• Methods for direct gene transfer
• Marker and reporter genes
• Promoters used in plant vectors
• Plant viral vectors
• Molecular techniques for analysis of transgenics (copy number
• transgene stability
• Marker-free transgenics and environmental
• social and legal issues associated with transgenic plants
• Case studies for genetic engineering in plants for traits of agronomic value
• abiotic stresses and herbicide tolerance

Unit 7 Molecular Farming

• Transgenic crops for production of antibodies
• viral antigens and peptide hormones in plants
• Edible vaccines and Nutraceuticals
• Plant Biotechnology for biofuels 5 Subject Code: 3170414 w.e.f

Unit 8 Genome Editing

6 lecture hours

• The history of targeted mutations in plants
• Use of ZFNs and TALENs as early tools for genome editing
• Discovery of CRISPR-Cas system and its applications
• Recent innovations in the technology and case studies where CRISPRCas has been used for plant improvement