Unit 1 Introduction to Enzymes

• Classification of enzymes
• Mechanisms of enzyme action
• concept of active site and energetics of enzyme substrate complex formation
• specificity of enzyme action
• principles of catalysis – collision theory
• transition state theory
• role of entropy in catalysis. 7 10.41

Unit 2 Kinetics of Enzyme Action

• Kinetics of single substrate reactions
• estimation of Michelis – Menten parameters
• multi substrate reactions- mechanisms and kinetics
• turnover number
• types of inhibition & models –substrate
• Allosteric regulation of enzymes
• Monod Changeux Wyman model
• pH and temperature effect on enzymes & deactivation kinetics. 9 14.58

Unit 3 Enzyme Immobilization

• Physical and chemical techniques for enzyme immobilization – adsorption
• matrix entrapment
• covalent binding etc.
• advantages and disadvantages
• Analysis of Film and Pore Diffusion Effects on Kinetics of immobilized Enzyme Reactions
• Formulation of dimensionless group and calculation of Effectiveness Factors. 8 12.50

Unit 4 Purification & Characterization of Enzymes from Natural Sources

2 lecture hours

• Production and purification of crude enzyme extracts from plant
• animal and microbial sources
• methods of characterization of enzymes
• development of enzymatic assays
• Commercial application of enzymes in food
• pharmaceutical and other industries
• Enzymes for analytical and diagnostic applications. 8 12.50