Unit 1 Basic Techniques

• Agarose gel electrophoresis
• Nucleic acid blotting: Southern
• western and northern blotting, PCR, Competent cell preparation
• transformation of E. coli
• Advance Techniques in gene expression and analysis: RT-PCR
• Real-Time PCR, microarray. 10 Program Name: Bachelor of Engineering Level: UG Branch: Biotechnology Subject Code: BE05004031 Subject Name: Genetic Engineering

Unit 2 Cutting and joining DNA molecules

10 lecture hours

• various restriction enzymes
• Cloning Vectors: Plasmids and Phage Vectors
• Phasmids and other advanced vectors for recombinant protein production and purification
• animal and plant vector
• Introducing DNA into bacterial cells
• cloning in bacteria
• animal and plant cells
• recombinant clone selection
• transient and inducible expression system

Unit 3 Genomic libraries and sequencing

10 lecture hours

• DNA and cDNA library preparation
• cloning strategies for library preparation
• screening of libraries
• Sequencing: DNA sequencing
• concept of next generation sequencing technology
• whole genome sequencing
• analyzing sequence data
• mapping and identification coding and non-coding gene
• transgenic animals and plants
• molecular pharming

Unit 4 Transgenics and Molecular Pharming

10 lecture hours

• The creation and use of genetically modified organisms (GMOs) for research or agricultural purposes
• including the introduction of foreign genes into animal or plant genomes
• Molecular Pharming: The use of genetically modified organisms (such as plants
• or bacteria) to produce pharmaceutical proteins or vaccines

Unit 5 Advanced genomics

5 lecture hours

• CRISPR/Cas9 Gene Editing
• Single-Cell RNA Sequencing
• Gene Drives: Gene Therapy: Synthetic Genomics