Unit 1 Introduction to Bioprocessing

• Role of a bioprocess engineer
• kinetics of microbial growth
• substrate utilization, and product formation
• Batch and continuous culture. 5 Program Name: Bachelor of Engineering Level: UG Branch: Biotechnology Subject Code: BE05004021 Subject Name: Bioprocess Engineering

Unit 2 Sterilization

5 lecture hours

• Media sterilization
• kinetics of thermal death of cells & spores
• design of batch and continuous thermal sterilization
• sterilization of air and filter design
• radiation and chemical sterilization

Unit 3 Biofluid mechanics

5 lecture hours

• Newtonian and non-Newtonian fluids
• factors affecting broth viscosity
• velocity distribution in laminar flow and turbulent flow
• aeration and agitation
• power requirement for gassed and un-gassed systems
• Rate of mixing

Unit 4 Bioprocess Heat Transfer

10 lecture hours

• Modes of heat transfer
• Steady-state and un-steady-state heat transfer
• Heat transfer coefficients
• Boiling and condensation heat transfer
• Heat transfer equipment

Unit 5 Mass Transfer in Bioreactors

10 lecture hours

• Theories of mass transfer
• Gas-Liquid mass transfer
• Measurement of KLa, Oxygen transfer methodology
• Maximum cell concentration

Unit 6 Applications of bioreactors

10 lecture hours

• On-line and off-line measurement in bioreactors
• Various types of microbial and enzyme reactors
• Scale-Up in Bioprocessing: Challenges and strategies in scaling up bioprocesses from laboratory to industrial scale
• including issues related to heat and mass transfer
• shear forces, and mixing
• Bioprocess Economics and Cost Optimization: Analysis of the economic aspects of bioprocessing
• including cost factors such as raw materials
• energy consumption, and labor