Advanced Molecular Biology
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Advanced Molecular Biology Syllabus — Unit-wise Content
The official GTU curriculum for Advanced Molecular Biology (subject code 3150401) as prescribed for Biotech · Semester 5 divides the course into 5 units, covering 85 listed topics. The unit-wise breakdown below is read from the GTU syllabus document for this subject code, so you can check what the course actually covers before deciding which papers to attempt.
Download the official syllabus PDF (3150401)Unit 1 Regulation of Transcription
- Prokaryotes : Gene regulation: Repressors
- positive and negative regulation
- Constitutive and Inducible
- small molecule regulators
- operon concept: lac, trp, his operons
- anti- termination
- stringent control
- translational control
- DNA re- arrangement
- two component system
- regulatory RNA – riboswitch
- antisense RNA
- transcriptional control in lambda phage
- Eukaryotes: Regulation of RNA processing: capping
- mRNA stability and degradation: degradation and surveillance pathways
- RNA editing
- Nuclear export of mRNA
- Catalytic RNA: Group I and Group II introns splicing
- Peptidyl transferase
- Regulatory RNA and RNA interference mechanisms
- non-coding RNA
- Silencers and insulators
- Subject Code: 3150401 w.e.f
- AY 2018-19 mechanism of silencing and activation
- histone modifications and chromatin remodeling
- Methods for studying DNA-protein interaction: EMSA
- DNase I footprinting
- methylation interference assay
- chromatin immunoprecipitation
Unit 2 Genetic Engineering
- General requirements for performing a genetic engineering experiment
- biology of restriction
- types and properties of restriction endonucleases and methylases
- DNA ligase
- Klenow enzyme
- T4 DNA polymerase
- polynucleotide kinase
- alkaline phosphatase
- cohesive and blunt end ligation
- homopolymeric tailing
- labelling of DNA: nick translation
- random priming
- Biology of plasmids and bacteriophages
- Plasmids & M13 mp vectors
- pUC19 series and Bluescript vectors
- Lambda vectors
- Insertion and Replacement vectors
- Artificial chromosome vectors (YACs
- Principles for maximizing gene expression (RNA and protein)
- protein expression vectors
- pET-based vectors
- Protein purification
- MBP-tag etc
- Intein- based vectors
- Inclusion bodies
- methodologies to reduce formation of inclusion bodies
- protein refolding
- mammalian expression and replicating vectors
- Baculovirus and Pichia vector system
- wheat germ line expression system
- plant based vectors
- Ti and Ri as vectors
- yeast vectors
- shuttle vectors
Unit 3 Gene silencing and genome editing technologies
- Gene silencing techniques
- introduction to siRNA
- siRNA technology
- Micro RNA
- construction of siRNA vectors
- principle and application of gene silencing
- gene knockouts and gene therapy
- creation of transgenic plants
- introduction to methods of genetic manipulation in different model systems e.g. fruit flies (Drosophila)
- worms (C. elegans), and mouse model Transgenics - gene replacement
- gene targeting
- creation of transgenic and knock-out mice
- disease model
- introduction to genome editing by CRISPR-CAS with specific emphasis on Chinese and American clinical trials
Unit 4 Molecular mapping and marker assisted selection
- Molecular markers - hybridization and PCR based markers RFLP
- RAPD, STS, SSR, AFLP
- SNP markers
- DNA fingerprinting- principles and applications
- introduction to mapping of genes/QTLs
- marker-assisted selection - strategies for Introducing genes of biotic and abiotic stress resistance in plants: genetic basis for disease resistance in animals
- molecular diagnostics of pathogens in plants and animals
- detection of meat adulteration using DNA based methods
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