Animal and Plant Biotechnology
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Follow on Instagram for UpdatesAnimal and Plant Biotechnology Syllabus — Unit-wise Content
The official GTU curriculum for Animal and Plant Biotechnology (subject code 2180402) as prescribed for Biotech · Semester 8 divides the course into 5 units, covering 72 listed topics. The unit-wise breakdown below is read from the GTU syllabus document for this subject code, so you can check what the course actually covers before deciding which papers to attempt.
Download the official syllabus PDF (2180402)Unit 1 UNIT I
- Animal Cell and Tissue Culture History and scope of animal cell and tissue culture
- advantages and disadvantages of tissue culture
- laboratory facilities for tissue culture
- the substrate on which cells grow
- treatment of substrate surfaces
- feeder layers on substrate
- the gas phase for tissue culture
- culture media for cells and tissues
- culture procedures
- Disaggregation (enzymatic and mechanical) of tissue and primary culture
- cultured cells and evolution of cell lines
- maintenance of culture-cell lines
- Tissue culture (slide
- flask and test tube cultures)
- organ culture
- whole embryo culture
- tissue engineering (artificial skin and artificial cartilage). 11 22.91
Unit 2 Unit II
- In Vitro Fertilization & Transgenic Animals In vitro fertilization (IVF) in humans
- embryo transfer (ET) in humans
- IVF and embryo culture in farm animals (e.g. cow)
- embryo transfer in cattle
- Gene transfer or transfection (using eggs and cultured stem cells)
- targeted gene transfer
- transgenic animals
Unit 3 UNIT III
- Plant tissue culture Introduction
- Laboratory organization
- Nutrition medium
- media composition
- different kinds of sterilization techniques for the preparation of sterile media
- containers & instruments
- maintenance of aseptic conditions. 5 10.41
Unit 4 Unit IV
- Types of culture Cytodifferentiation
- Organogenic differentiation
- Types of culture - Seed culture
- Embryo culture
- Mature embryo culture
- Immature embryo culture/embryo rescue
- Application of embryo culture
- Callus culture
- Organ culture
- Nucellus culture
- Application Endosperm culture
- Application cell culture
- secondary metabolite production
- haploid production
- protoplast isolation & fusion
- somaclonal variation. 12 25.00
Unit 5 Unit V
- Transgenics in crop improvement and Bioethics Resistance of biotic stresses
- Insect resistance
- Resistance genes from microorganisms
- Resistance genes from higher plants
- Resistance genes from animals
- Virus resistance
- Coat protein mediated cross protection
- Non structural protein mediated resistance
- Antisense and sense mediated resistance
- Satellite RNA protection
- Pathogen targeted resistance
- Disease resistance
- Pathogenesis related proteins
- Manipulation of disease resistance genes
- Resistance of abiotic stresses
- Herbicide resistance
- Transgenic for quality
- Transgenic for improved storage
- Longer life transgenic flowers
- Transgenic for male sterility
- Terminator technology for use in hybrid seed production
- Commercial transgenic crops
- Bioethics: Concepts of bioethics
- socioeconomic impacts of ethical and non-ethical practices
- ethical aspects of genetically modified organisms and their release in environment. 15 31.25
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