Proteins and enzymes
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Proteins and enzymes Syllabus — Unit-wise Content
The official GTU curriculum for Proteins and enzymes (subject code 3160413) as prescribed for Biotech · Semester 6 divides the course into 3 units, covering 23 listed topics. The unit-wise breakdown below is read from the GTU syllabus document for this subject code, so you can check what the course actually covers before deciding which papers to attempt.
Download the official syllabus PDF (3160413)Unit 1 Introduction to Enzymes
- Classification of enzymes
- Mechanisms of enzyme action
- concept of active site and energetics of enzyme substrate complex formation
- specificity of enzyme action
- principles of catalysis – collision theory
- transition state theory
- role of entropy in catalysis
Unit 2 Kinetics of Enzyme Action
- Kinetics of single substrate reactions
- estimation of Michelis – Menten parameters
- multi substrate reactions- mechanisms and kinetics
- turnover number
- types of inhibition & models –substrate
- Allosteric regulation of enzymes
- Monod Changeux Wyman model
- pH and temperature effect on enzymes & deactivation kinetics
Unit 3 Enzyme Immobilization
- Physical and chemical techniques for enzyme immobilization – adsorption
- matrix entrapment
- covalent binding etc.
- advantages and disadvantages
- Analysis of Film and Pore Diffusion Effects on Kinetics of immobilized Enzyme Reactions
- Formulation of dimensionless group and calculation of Effectiveness Factors
- Applied Enzyme Catalysis (Applications of Hydrolytic Enzymes
- Other Applications of Enzymes in Solution) Subject Code: 3160413 Wef 2018-19
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